Foxp3^tm11.2Ayr
Targeted Allele Detail

Summary

• Symbol: Foxp3^tm11.2Ayr
• Name: forkhead box P3; targeted mutation 11.2, Alexander Y Rudensky
• MGI ID: MGI:6711582
• Synonyms: CNS0^delta, Foxp3^{deltaCNS0-GFP}
• Gene: Foxp3 Location: ChrX:7445915-7461482 bp, + strand Genetic Position: ChrX, 3.41 cM
• Alliance: Foxp3^tm11.2Ayr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:305560
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Modified regulatory region)
• Mutations: Insertion, Intragenic deletion
• Foxp3^tm11.2Ayr involves 2 genes/genome features (Ppp1r3f, Rr122) View all
• Mutation details: A GFP reporter gene was inserted in-frame with the 5' end of the gene's coding sequence. The upstream conserved non-coding sequence 0 (CNS0 (Rr122), a STAT5-binding enhancer located at chrX:7436913-7437513 (GRCm39) within intron 1 of Ppp1r3f) was flanked upstream by a loxP site and downstream by an FRT site flanked neomycin resistance gene cassette and a second loxP site. Flp-mediated recombination removed the neo cassette. Cre-mediated recombination removed CNS0. (J:305560)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Foxp3 Mutation: 57 strains or lines available

References

• Original: J:305560 Dikiy S, et al., A distal Foxp3 enhancer enables interleukin-2 dependent thymic Treg cell lineage commitment for robust immune tolerance. Immunity. 2021 May 11;54(5):931-946.e11
• All: 2 reference(s)