Mup3em1(cre/ERT2)Hzhu
Endonuclease-mediated Allele Detail
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Symbol: |
Mup3em1(cre/ERT2)Hzhu |
Name: |
major urinary protein 3; endonuclease-mediated mutation 1, Hao Zhu |
MGI ID: |
MGI:6704916 |
Synonyms: |
Mup3-CreER |
Gene: |
Mup3 Location: Chr4:62001713-62005554 bp, - strand Genetic Position: Chr4, 32.99 cM
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Alliance: |
Mup3em1(cre/ERT2)Hzhu page
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Allele Type: |
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Endonuclease-mediated (Inducible, Recombinase) |
Inducer: |
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tamoxifen |
Mutation: |
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Insertion
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Mup3em1(cre/ERT2)Hzhu expression driven by
1 gene
Knock-in expression driven by:
Organism |
Driver Gene |
Note |
mouse |
Mup3 (MGI:97235) |
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Mutation details: The allele was generated using CRISPR/Cas9 endonuclease-mediated genome editing to have an IRES enhancer sequence and a CreER(T2) fusion gene inserted immediately downstream of the mouse Mup3 gene. These sequences and Cas9 nuclease were introduced into single-cell C57BL/6J zygotes and transferred to pseudopregnant females. Progeny were screened by DNA sequencing to identify correctly targeted pups, which were then bred to C57BL/6J mice for germline transmission.
(J:304477)
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Activity: |
Tissue activity of this recombinase allele
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Driver:
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Mup3
(mouse)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Mup3 Mutation: |
10 strains or lines available
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Original: |
J:304477 Wei Y, et al., Liver homeostasis is maintained by midlobular zone 2 hepatocytes. Science. 2021 Feb 26;371(6532) |
All: |
2 reference(s) |
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