Igh^{tm4.1(IGH,Adam6a,Adam6b)Vlcg}
Targeted Allele Detail

Summary

• Symbol: Igh^{tm4.1(IGH,Adam6a,Adam6b)Vlcg}
• Name: immunoglobulin heavy chain complex; targeted mutation 4.1, Velocigene
• MGI ID: MGI:6696096
• Synonyms: Adam6^res
• Gene: Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:277644
• Parent Cell Line: F1H4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NTac)F1

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion
• Igh^{tm4.1(IGH,Adam6a,Adam6b)Vlcg} expresses 3 genes
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Adam6a (MGI:2676316)
  mouse	Adam6b (MGI:2444636)
  human	IGH (3492)

• Mutation details: Retargeting of the human replacement of Igh, replaced the human Adam6 pseudogene with the mouse genes separated by an FRT-flanked hygromycin resistance cassette. Flp-mediated recombination removed the selection cassette. (J:277644)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igh Mutation: 43 strains or lines available

References

• Original: J:277644 Voronina VA, et al., Deletion of Adam6 in Mus musculus leads to male subfertility and deficits in sperm ascent into the oviduct. Biol Reprod. 2019 Mar 1;100(3):686-696
• All: 1 reference(s)