Gt(ROSA)26Sor^{tm4(CAG-Irf3*S388D*S390D)Evdr}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm4(CAG-Irf3*S388D*S390D)Evdr}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 4, Evan Rosen
• MGI ID: MGI:6695703
• Synonyms: IRF3-2D
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm4(CAG-Irf3*S388D*S390D)Evdr} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:304593
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Conditional ready, Epitope tag, Inserted expressed sequence)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm4(CAG-Irf3*S388D*S390D)Evdr} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Irf3 (MGI:1859179)	carries S388D and S390D amino acid substitutions

• Mutation details: The targeting vector contains from 5' to 3', a chicken beta-actin promoter, a loxP-flanked stop cassette, the 3XFlag-tagged mouse Irf3 sequence modified to carry S388D and S390D amino acid substitutions (IRF3-2D), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), and a bovine growth hormone polyA signal (bGHpA). Therefore the final construct reads ROSA26::CAG::loxP-STOP-loxP::3XFlag-IRF3-2D::WPRE::bGHpA. The S388D and S390D mutations result in a constitutively active allele without the need for an external stimulus (ie. lipopolysaccharide, poly I:C). (J:304593)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 944 strains or lines available

References

• Original: J:304593 Yan S, et al., IRF3 reduces adipose thermogenesis via ISG15-mediated reprogramming of glycolysis. J Clin Invest. 2021 Apr 1;131(7)
• All: 1 reference(s)