Phf7^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Phf7^{tm1c(EUCOMM)Hmgu}
• Name: PHD finger protein 7; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6690457
• Synonyms: Phf7^flox
• Gene: Phf7 Location: Chr14:30959646-30973274 bp, - strand Genetic Position: Chr14, 19.09 cM
• Alliance: Phf7^{tm1c(EUCOMM)Hmgu} page
• IMPC: Phf7 gene page

Mutation origin

• Mutant Cell Line: HEPD0568_1_A09
• Germline Transmission: Earliest citation of germline transmission: J:298823
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 30962798 of Chromosome 14 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 30964146. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:298823)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Phf7 Mutation: 27 strains or lines available

References

• Original: J:298823 Kim CR, et al., PHF7 Modulates BRDT Stability and Histone-to-Protamine Exchange during Spermiogenesis. Cell Rep. 2020 Jul 28;32(4):107950
• All: 1 reference(s)