Gt(ROSA)26Sor^{tm3(CAG-Dgkz*G355D/GFP)Xpz}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm3(CAG-Dgkz*G355D/GFP)Xpz}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 3, Xiaoping Zhong
• MGI ID: MGI:6690447
• Synonyms: GFP-DGKzeta^KD
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm3(CAG-Dgkz*G355D/GFP)Xpz} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:298856
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Conditional ready, Epitope tag, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm3(CAG-Dgkz*G355D/GFP)Xpz} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Dgkz (MGI:1278339)

• Mutation details: The targeting construct introduced into the locus a ubiquitous CAG promoter, floxed STOP cassette, EGFP reporter gene fused to the kinase dead version of the mouse gene (glycine residue in the ATP binding domain mutated to alanine; G355D), IRES and human CD2 epitope tag. (J:298856)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1062 strains or lines available

References

• Original: J:298856 Xie D, et al., Negative control of diacylglycerol kinase zeta-mediated inhibition of T cell receptor signaling by nuclear sequestration in mice. Eur J Immunol. 2020 Nov;50(11):1729-1745
• All: 1 reference(s)