Gpr139^{tm1.1(flpo)Tasic}
Targeted Allele Detail

Summary

• Symbol: Gpr139^{tm1.1(flpo)Tasic}
• Name: G protein-coupled receptor 139; targeted mutation 1.1, Bosiljka Tasic
• MGI ID: MGI:6514363
• Synonyms: Gpr139^{tm1.1(flpo)Hze}
• Gene: Gpr139 Location: Chr7:118739970-118783761 bp, - strand Genetic Position: Chr7, 63.79 cM
• Alliance: Gpr139^{tm1.1(flpo)Tasic} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Gpr139^{tm1.1(flpo)Tasic} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Gpr139 (MGI:2685341)

• Mutation details: The targeting vector contained, from 5' to 3', a partial Gpr139 sequence within exon 2 up to and including the endogenous stop codon, an internal ribosome entry site 2 sequence (IRES2; allows translation initiation in the middle of an mRNA sequence), a FlpO recombinase gene (a codon-optimized FLPe gene-modified for translation in mammalian cells and higher recombination efficiency in cells and in mice), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a bovine growth hormone polyA sequence, an AttB site, a PGK/gb2 promoter-Neomycin resistance gene-PGK polyA cassette and an AttP site. Chimeric mice were bred to PhiC31-expressing mice to remove the AttB/AttP-flanked sequences (PGK/gb2-Neo-pA) and replace it with the recombined AttB/AttP site (AttL). (J:101977)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Gpr139 (mouse) Summary of all recombinase alleles driven by Gpr139. Alliance mouse Gpr139 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gpr139 Mutation: 27 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 3 reference(s)