Lgr5^{tm2.1(Hbegf)Niba}
Targeted Allele Detail

Summary

• Symbol: Lgr5^{tm2.1(Hbegf)Niba}
• Name: leucine rich repeat containing G protein coupled receptor 5; targeted mutation 2.1, Nick Barker
• MGI ID: MGI:6507039
• Synonyms: Lgr5-2A-DTR
• Gene: Lgr5 Location: Chr10:115286219-115423685 bp, - strand Genetic Position: Chr10, 63.51 cM
• Alliance: Lgr5^{tm2.1(Hbegf)Niba} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:283792
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Lgr5^{tm2.1(Hbegf)Niba} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  Not Specified	Hbegf

• Mutation details: Sequence for the self-cleaving A2 peptide, a diphtheria toxin receptor (HBEGF/DTR) cDNA and a loxP site flanked neomycin resistance gene cassette were inserted immediately upstream of the endogenous stop codon. The neo cassette was removed through subsequent cre-mediated recombination. This allele expresses the endogenous peptide and the inserted DTR peptide. (J:283792)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lgr5 Mutation: 58 strains or lines available

References

• Original: J:283792 Seishima R, et al., Neonatal Wnt-dependent Lgr5 positive stem cells are essential for uterine gland development. Nat Commun. 2019 Nov 26;10(1):5378
• All: 6 reference(s)