Tnni3k^em4Hsv
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tnni3k^em4Hsv
• Name: TNNI3 interacting kinase; endonuclease-mediated mutation 4, Henry M Sucov
• MGI ID: MGI:6506814
• Synonyms: I685T
• Gene: Tnni3k Location: Chr3:154491928-154761044 bp, - strand Genetic Position: Chr3, 79.32 cM
• Alliance: Tnni3k^em4Hsv page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Humanized sequence)
• Mutation: Nucleotide substitutions
• Mutation details: CRISPR/Cas9 technology generated an isoleucine (ATC) codon to a threonine (ACA) codon at position 685 (I685T) in exon 21. An additional base change in the third position of the following glycine codon was introduced to eliminate the Cas9 PAM sequence to prevent retargeting. (J:300000)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tnni3k Mutation: 47 strains or lines available

References

• Original: J:300000 Gan P, et al., The prevalent I686T human variant and loss-of-function mutations in the cardiomyocyte-specific kinase gene TNNI3K cause adverse contractility and concentric remodeling in mice. Hum Mol Genet. 2021 Jan 6;29(21):3504-3515
• All: 1 reference(s)