Igh^{tm3.1(Igl-2MOPC315*)Bbo}
Targeted Allele Detail

Summary

• Symbol: Igh^{tm3.1(Igl-2MOPC315*)Bbo}
• Name: immunoglobulin heavy chain complex; targeted mutation 3.1, Bjarne Bogen
• MGI ID: MGI:6506427
• Synonyms: Vlambda^315m
• Gene: Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:282821
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: The targeting vector replaced the D-Q52*01-J4 with the B1-8 promoter and the modified V-region of the M315 myeloma protein heavy chain (generated from the MOPC315 cell line) in which the Vlambda2 exon 2 was replaced with a synthetic Vlambda315 exon 2 containing the modification YST to FRN (residues 94-96; TACAGCACC to TTCAGAAAC) and an FRT-flanked neomycin resistance cassette. Flp-mediated recombination removed the selection cassette. (J:282821)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igh Mutation: 47 strains or lines available

Notes

ES cells = BL1 (BALB/c).

References

• Original: J:282821 Huszthy PC, et al., B cell receptor ligation induces display of V-region peptides on MHC class II molecules to T cells. Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25850-25859
• All: 1 reference(s)