C4b^em1Crr
Endonuclease-mediated Allele Detail

Summary

• Symbol: C4b^em1Crr
• Name: complement C4B (Chido blood group); endonuclease-mediated mutation 1, Michael C Carroll
• MGI ID: MGI:6505588
• Synonyms: C4A
• Gene: C4b Location: Chr17:34947354-34962856 bp, - strand Genetic Position: Chr17, 18.29 cM
• Alliance: C4b^em1Crr page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Humanized sequence)
• Mutation: Nucleotide substitutions
• Mutation details: Using sgRNAs and ssODN templates with CRISPR/Cas9 technology, the gene was changed to make the isotypic region resemble the human C4A gene: endogenous codon sequence for PCPVIH was changed to PCPVLD (p.I1120_H1121delLDins) (J:301308)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any C4b Mutation: 106 strains or lines available

References

• Original: J:301308 Simoni L, et al., Complement C4A Regulates Autoreactive B Cells in Murine Lupus. Cell Rep. 2020 Nov 3;33(5):108330
• All: 1 reference(s)