Wnt1^tm1.1Kiv
Targeted Allele Detail

Summary

• Symbol: Wnt1^tm1.1Kiv
• Name: wingless-type MMTV integration site family, member 1; targeted mutation 1.1, Riku Kiviranta
• MGI ID: MGI:6505569
• Synonyms: Wnt1^fl
• Gene: Wnt1 Location: Chr15:98687738-98691711 bp, + strand Genetic Position: Chr15, 54.65 cM, cytoband F1-F3
• Alliance: Wnt1^tm1.1Kiv page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:284679
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The targeting vector, PRPGS00167_B-B10 obtained from the European Conditional Mouse Mutagenesis Program (EUCOMM) inserted a cassette composed of an FRT site followed by lacZ sequence and a loxP site into the first intron between exon 1 and 2. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site, and a second loxP site. A third loxP site is inserted in exon 4. The exons 2-3 and a part of exon 4 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exons 2-4. (J:284679)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Wnt1 Mutation: 29 strains or lines available

References

• Original: J:284679 Wang F, et al., Mesenchymal Cell-Derived Juxtacrine Wnt1 Signaling Regulates Osteoblast Activity and Osteoclast Differentiation. J Bone Miner Res. 2019 Jun;34(6):1129-1142
• All: 3 reference(s)