App^tm1.1Ehk
Targeted Allele Detail

Summary

• Symbol: App^tm1.1Ehk
• Name: amyloid beta precursor protein; targeted mutation 1.1, Edward H Koo
• MGI ID: MGI:6505479
• Synonyms: APP D664A KI
• Gene: App Location: Chr16:84751236-84972187 bp, - strand Genetic Position: Chr16, 46.92 cM, cytoband C3-qter
• Alliance: App^tm1.1Ehk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:301251
• Parent Cell Line: iTL IC1 (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: An AC-to-CT mutation (GT-to-AG on forward strand) was engineered in exon 18, which changes aspartic acid codon 664 to an alanine codon (p.D664A). This mutation prevents cleavage of the encoded peptide at the aspartate residue by caspases or caspase-like proteases. The loxP and FRT site flanked neomycin resistance gene cassette that was inserted downstream of exon 18 was deleted through subsequent flp-mediated recombination. (J:301251)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any App Mutation: 111 strains or lines available

References

• Original: J:301251 Park G, et al., Caspase Activation and Caspase-Mediated Cleavage of APP Is Associated with Amyloid beta-Protein-Induced Synapse Loss in Alzheimer's Disease. Cell Rep. 2020 Jun 30;31(13):107839
• All: 1 reference(s)