Cyp24a1^{tm1d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Cyp24a1^{tm1d(KOMP)Wtsi}
• Name: cytochrome P450, family 24, subfamily a, polypeptide 1; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:6505436
• Synonyms: Cyp24a1^-
• Gene: Cyp24a1 Location: Chr2:170324877-170339065 bp, - strand Genetic Position: Chr2, 91.91 cM
• Alliance: Cyp24a1^{tm1d(KOMP)Wtsi} page
• IMPC: Cyp24a1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:295151
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 170334283 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 170335095. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:295151)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cyp24a1 Mutation: 29 strains or lines available

References

• Original: J:295151 Sheng L, et al., Mammary-specific ablation of Cyp24a1 inhibits development, reduces proliferation and increases sensitivity to vitamin D. J Steroid Biochem Mol Biol. 2019 May;189:240-247
• All: 1 reference(s)