Col1a1^{tm1(tetO-GFP/RNAi:Atg5)Mnar}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm1(tetO-GFP/RNAi:Atg5)Mnar}
• Name: collagen, type I, alpha 1; targeted mutation 1, Masashi Narita
• MGI ID: MGI:6504237
• Synonyms: Atg5_1065
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm1(tetO-GFP/RNAi:Atg5)Mnar} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:289254
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inducible, Knockdown, RMCE-ready, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: RMCE (recombinase-mediated cassette exchange) was utilized to insert tetO-GFP/RNAi:Atg5 into the Col1a1 locus. D34 embryonic stem cells, expressing CAG-rtTA3 knocked into the Rosa26 locus, were used. The vector contains a tetracycline responsive promoter (TRE;tetO) controlling expression of a GFP reporter and short-hairpin RNA sequence targeting Atg5 embedded in the miR-E design (an improved design variant of Mir30 structure). (J:289254)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 166 strains or lines available

References

• Original: J:289254 Cassidy LD, et al., A novel Atg5-shRNA mouse model enables temporal control of Autophagy in vivo. Autophagy. 2018;14(7):1256-1266
• All: 3 reference(s)