Gt(ROSA)26Sor^em2Mvw
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^em2Mvw
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 2, Michael Wiles
• MGI ID: MGI:6504045
• Synonyms: B6.RosaBxb-GT
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^em2Mvw page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Not Specified)
• Mutation: Insertion
• Mutation details: CRISPR-mediated NHEJ insertion of a 48 base pair duplex DNA oligo with no homology arms introduced a single Bxb1 AttP site in the forward orientation, and during endogenous DNA repair an indel of 3 bp (GAT) and + 6 bp (CAAACC) occurred just upstream of the engineered insertion. (J:299754)
• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 942 strains or lines available

References

• Original: J:299754 Farris MH, et al., Detection of CRISPR-mediated genome modifications through altered methylation patterns of CpG islands. BMC Genomics. 2020 Dec 2;21(1):856
• All: 2 reference(s)