Gt(ROSA)26Sor^{tm1.1(Cas9)Rrad}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1.1(Cas9)Rrad}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Roland Rad
• MGI ID: MGI:6503895
• Synonyms: Rosa26^Cas9
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1.1(Cas9)Rrad} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:273279
• Parent Cell Line: JM8 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Endonuclease)
• Mutation: Insertion
• Mutation details: A human codon-optimized hemagglutinin-tagged Cas9 sequence derived from Streptococcus pyogenes was inserted into the Gt(ROSA)26Sor locus with a loxP-flanked puromycin resistance-containing stop cassette preceding the Cas9 sequence. This constitutively expressing allele was generated by deletion of the loxP-flanked stop cassette in the germline. (J:273279)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1060 strains or lines available

References

• Original: J:273279 Weber J, et al., PiggyBac transposon tools for recessive screening identify B-cell lymphoma drivers in mice. Nat Commun. 2019 Mar 29;10(1):1415
• All: 2 reference(s)