A930004D18Rik^{tm1b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: A930004D18Rik^{tm1b(EUCOMM)Wtsi}
• Name: RIKEN cDNA A930004D18 gene; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:6502674
• Synonyms: tm1b
• Gene: A930004D18Rik Location: Chr2:18029998-18042552 bp, - strand Genetic Position: Chr2, 12.82 cM
• Alliance: A930004D18Rik^{tm1b(EUCOMM)Wtsi} page
• IMPC: A930004D18Rik gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:299775
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 18029716 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 18033340. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:299775)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any A930004D18Rik Mutation: 32 strains or lines available

References

• Original: J:299775 Xie C, et al., Dedicated transcriptomics combined with power analysis lead to functional understanding of genes with weak phenotypic changes in knockout lines. PLoS Comput Biol. 2020 Nov;16(11):e1008354
• All: 1 reference(s)