Hmgcr^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Hmgcr^{tm1c(KOMP)Wtsi}
• Name: 3-hydroxy-3-methylglutaryl-Coenzyme A reductase; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6502672
• Synonyms: Hmgcr^fl
• Gene: Hmgcr Location: Chr13:96785475-96807444 bp, - strand Genetic Position: Chr13, 50.65 cM
• Alliance: Hmgcr^{tm1c(KOMP)Wtsi} page
• IMPC: Hmgcr gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:299774
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 96801026 of Chromosome 13 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 96801803. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:299774)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hmgcr Mutation: 62 strains or lines available

References

• Original: J:299774 De Giorgi M, et al., Depletion of essential isoprenoids and ER stress induction following acute liver-specific deletion of HMG-CoA reductase. J Lipid Res. 2020 Dec;61(12):1675-1686
• All: 2 reference(s)