Phactr1tm1d(KOMP)Wtsi
Targeted Allele Detail
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Symbol: |
Phactr1tm1d(KOMP)Wtsi |
Name: |
phosphatase and actin regulator 1; targeted mutation 1d, Wellcome Trust Sanger Institute |
MGI ID: |
MGI:6489962 |
Gene: |
Phactr1 Location: Chr13:42834099-43292002 bp, + strand Genetic Position: Chr13, 21.05 cM
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Alliance: |
Phactr1tm1d(KOMP)Wtsi page
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IMPC: |
Phactr1 gene page |
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Germline Transmission: |
Earliest citation of germline transmission:
J:299011
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Parent Cell Line: |
JM8A3.N1 (ES Cell)
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Strain of Origin: |
C57BL/6N-Atm1Brd
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Project Collection: |
KOMP-CSD
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Allele Type: |
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Targeted (Null/knockout) |
Mutation: |
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Intragenic deletion
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Mutation details: The L1L2_Bact_P cassette was inserted at position 43230654 of Chromosome 13 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 43231719. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml
(J:299011)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Phactr1 Mutation: |
43 strains or lines available
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Original: |
J:299011 Fedoryshchak RO, et al., Molecular basis for substrate specificity of the Phactr1/PP1 phosphatase holoenzyme. Elife. 2020 Sep 25;9:e61509 |
All: |
1 reference(s) |
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