Adtrp^em1Sagh
Endonuclease-mediated Allele Detail

Summary

• Symbol: Adtrp^em1Sagh
• Name: androgen dependent TFPI regulating protein; endonuclease-mediated mutation 1, Alan Saghatelian
• MGI ID: MGI:6488487
• Gene: Adtrp Location: Chr13:41916621-42001092 bp, - strand Genetic Position: Chr13, 20.61 cM
• Alliance: Adtrp^em1Sagh page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/cas9-mediated recombination created a 13 bp deletion in exon 2 upstream of the catalytic threonine (T47) resulting in a frameshift mutation and premature stop codon upstream of the catalytic threonine. Western blot analysis confirmed the absence of expression in brown adipose tissue from homozygous mice. (J:289837)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Adtrp Mutation: 21 strains or lines available

References

• Original: J:289837 Erikci Ertunc M, et al., AIG1 and ADTRP are endogenous hydrolases of fatty acid esters of hydroxy fatty acids (FAHFAs) in mice. J Biol Chem. 2020 May 1;295(18):5891-5905
• All: 1 reference(s)