Sdha^{tm2c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Sdha^{tm2c(KOMP)Wtsi}
• Name: succinate dehydrogenase complex, subunit A, flavoprotein (Fp); targeted mutation 2c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6488149
• Synonyms: Sdha^fl
• Gene: Sdha Location: Chr13:74470374-74498359 bp, - strand Genetic Position: Chr13, 40.15 cM
• Alliance: Sdha^{tm2c(KOMP)Wtsi} page
• IMPC: Sdha gene page

Mutation origin

• Mutant Cell Line: EPD0670_1_C11
• Germline Transmission: Earliest citation of germline transmission: J:320199
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Pgk_P
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 74485247 of Chromosome 13 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 74486138. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:320199)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sdha Mutation: 46 strains or lines available

References

• Original: J:320199 Kumar R, et al., A redox cycle with complex II prioritizes sulfide quinone oxidoreductase-dependent H2S oxidation. J Biol Chem. 2021 Nov 19;298(1):101435
• All: 6 reference(s)