Cnr1^tm1Jmz
Targeted Allele Detail

Summary

• Symbol: Cnr1^tm1Jmz
• Name: cannabinoid receptor 1; targeted mutation 1, Jeffrey M Zigman
• MGI ID: MGI:6479961
• Gene: Cnr1 Location: Chr4:33924593-33948831 bp, + strand Genetic Position: Chr4, 16.28 cM
• Alliance: Cnr1^tm1Jmz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:298337
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A loxP-flanked transcriptional blocking cassette was inserted at a site 11 base pairs upstream of the translational start site of the gene in exon 2 using BAC recombineering techniques generating a null allele. The transcriptional blocking cassette includes a splice acceptor site from the mouse engrailed 2 gene, an SV40 poly(A), an SV40 enhancer, a neomycin resistance gene, two HSV-TK poly(A) signals, a synthetic poly(A) signal/transcriptional pause signal, another synthetic poly(A) signal, and a Myc-associated zinc finger protein-binding site. (J:298337)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cnr1 Mutation: 41 strains or lines available

References

• Original: J:298337 Mani BK, et al., Combined Loss of Ghrelin Receptor and Cannabinoid CB1 Receptor in Mice Decreases Survival but does not Additively Reduce Body Weight or Eating. Neuroscience. 2020 Nov 1;447:53-62
• All: 1 reference(s)