Gt(ROSA)26Sor^{tm12.1(Nphs1-EGFP)Maoh}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm12.1(Nphs1-EGFP)Maoh}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 12.1, Masato Ohtsuka
• MGI ID: MGI:6477391
• Synonyms: Nephrin-EGFP
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm12.1(Nphs1-EGFP)Maoh} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:253434
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: Injection-based targeted transgenesis (PITT) using Gt(ROSA)26Sor^tm1Maoh replaced the CFP reporter gene with a mouse promoter driving podocyte-specific expression of an EGFP reporter gene. Flp-mediated recombination removed the neomycin resistance cassette. A lox2272 site remains upstream of the promoter. (J:253434)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1095 strains or lines available

References

• Original: J:253434 Tsuchida J, et al., Establishment of Nephrin Reporter Mice and Use for Chemical Screening. PLoS One. 2016;11(6):e0157497
• All: 1 reference(s)