Micu1^tm1aNarl
Targeted Allele Detail

Summary

• Symbol: Micu1^tm1aNarl
• Name: mitochondrial calcium uptake 1; targeted mutation 1a, National Laboratory Animal Center
• MGI ID: MGI:6473466
• Gene: Micu1 Location: Chr10:59538385-59699956 bp, + strand Genetic Position: Chr10, 29.6 cM
• Alliance: Micu1^tm1aNarl page
• IMPC: Micu1 gene page

Mutation origin

• Mutant Cell Line: WCW_1401_017
• Germline Transmission: Earliest citation of germline transmission: J:297104
• Parent Cell Line: WCW (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: IMPC

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Pgk_P Vector Type: Not Applicable
• Mutation details: The L1L2_Bact_P cassette was inserted upstream of the critical exon 7. The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 8. The critical exons 7 and 8 are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:297104)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Micu1 Mutation: 44 strains or lines available

References

• Original: J:297104 National Laboratory Animal Center, National Laboratory Animal Center Website. 2020
• All: 1 reference(s)