Notch2em1Rko
Endonuclease-mediated Allele Detail
|
Symbol: |
Notch2em1Rko |
Name: |
notch 2; endonuclease-mediated mutation 1, Raphael Kopan |
MGI ID: |
MGI:6472982 |
Synonyms: |
N2R1934A, N2RA |
Gene: |
Notch2 Location: Chr3:97920854-98057683 bp, + strand Genetic Position: Chr3, 42.42 cM
|
Alliance: |
Notch2em1Rko page
|
|
Strain of Origin: |
Not Applicable
|
|
Allele Type: |
|
Endonuclease-mediated (Not Specified) |
Mutation: |
|
Nucleotide substitutions
|
|
|
Mutation details: Arginine codon 1934 wast targeted with a gRNA (GGCATCCAGATCGGTTACA) and a donor oligonucleotide using CRISPR/Cas9 technology. The resulting arginine to alanine mutation (p.R1934A) prevents dimerization of proteolytically created Notch intracellular domains (NICDs).
(J:296947)
|
|
|
Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
|
Carrying any Notch2 Mutation: |
97 strains or lines available
|
|
Original: |
J:296947 Kobia FM, et al., Notch dimerization and gene dosage are important for normal heart development, intestinal stem cell maintenance, and splenic marginal zone B-cell homeostasis during mite infestation. PLoS Biol. 2020 Oct;18(10):e3000850 |
All: |
1 reference(s) |
|