Hsd17b12^tm1.1Mpo
Targeted Allele Detail

Summary

• Symbol: Hsd17b12^tm1.1Mpo
• Name: hydroxysteroid (17-beta) dehydrogenase 12; targeted mutation 1.1, Matti Poutanen
• MGI ID: MGI:6472704
• Gene: Hsd17b12 Location: Chr2:93863042-93988254 bp, - strand Genetic Position: Chr2, 51.62 cM
• Alliance: Hsd17b12^tm1.1Mpo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:296856
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: Using EUCOMM targeting vector PGS00030_C_C05, the locus was targeted for conditional knockout. The vector inserts an FRT site flanked lacZ/neomycin resistance fusion gene cassette and a loxP site into intron 1 (chr2:94117916 on build GRCm38) and a second loxP site into intron 2 (chr2:94117132). The lacZ/neo cassette was removed through subsequent flp-mediated recombination, leaving a conditional-ready allele with exon 2 floxed. (J:296856)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hsd17b12 Mutation: 52 strains or lines available

References

• Original: J:296856 Heikela H, et al., Hydroxysteroid (17beta) dehydrogenase 12 is essential for metabolic homeostasis in adult mice. Am J Physiol Endocrinol Metab. 2020 Sep 1;319(3):E494-E508
• All: 2 reference(s)