Pfkfb3^tm1.1Pec
Targeted Allele Detail

Summary

• Symbol: Pfkfb3^tm1.1Pec
• Name: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3; targeted mutation 1.1, Peter Carmeliet
• MGI ID: MGI:6470752
• Synonyms: pfkfb3^Loxp
• Gene: Pfkfb3 Location: Chr2:11476241-11558882 bp, - strand Genetic Position: Chr2, 8.73 cM
• Alliance: Pfkfb3^tm1.1Pec page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:296552
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: A loxP site was inserted into intron 2 and a second loxP site and an FRT site flanked neomycin resistance gene cassette into intron 6. The neo cassette was removed through subsequent flp-mediated recombination, leaving a conditional-ready allele with exons 3-6 floxed. (J:296552)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pfkfb3 Mutation: 36 strains or lines available

References

• Original: J:296552 Zhang J, et al., Endothelial Lactate Controls Muscle Regeneration from Ischemia by Inducing M2-like Macrophage Polarization. Cell Metab. 2020 Jun 2;31(6):1136-1153.e7
• All: 2 reference(s)