Gt(ROSA)26Sor^tm2.1Hrr
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^tm2.1Hrr
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2.1, Hans-Reimer Rodewald
• MGI ID: MGI:6470648
• Synonyms: Rosa26^{PolyloxExpress}
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^tm2.1Hrr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:296555
• Parent Cell Line: JM8A3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: The targeting construct introduced into the locus a splice acceptor, FRT site, neomycin resistance cassette, polyadenylation sequence, FRT site, tdTomato, stop cassette, and a series of 20 reporter genes interspersed with ten loxP sites in alternating orientation spaced 178 bp apart. The intervening nine DNA blocks are composed of unique sequences based on the AT2G21770 gene from Arabidopsis thaliana.. Flp-mediated recombination removed the selection cassette. (J:296555)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1096 strains or lines available

References

• Original: J:296555 Pei W, et al., Resolving Fates and Single-Cell Transcriptomes of Hematopoietic Stem Cell Clones by PolyloxExpress Barcoding. Cell Stem Cell. 2020 Sep 3;27(3):383-395.e8
• All: 2 reference(s)