Kras^tm1Kshn
Targeted Allele Detail

Summary

• Symbol: Kras^tm1Kshn
• Name: Kras proto-oncogene, GTPase; targeted mutation 1, Kevin Shannon
• MGI ID: MGI:6469949
• Synonyms: Kras^LSL-T58I
• Gene: Kras Location: Chr6:145162425-145195965 bp, - strand Genetic Position: Chr6, 77.37 cM, cytoband G2
• Alliance: Kras^tm1Kshn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:296368
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence, Null/knockout)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A loxP site flanked STOP cassette, with a 5' puromycin resistance gene cassette, was inserted into intron 2. Endogenous exon 3 was modified to harbor a C-to-T mutation at codon 58, resulting in a threonine to isoleucine mutation (p.T58I) associated with patients with Noonan syndrome (NS). (J:296368)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Kras Mutation: 69 strains or lines available

References

• Original: J:296368 Wong JC, et al., KrasP34R and KrasT58I mutations induce distinct RASopathy phenotypes in mice. JCI Insight. 2020 Sep 29
• All: 1 reference(s)