Piwil4^em1Doca
Endonuclease-mediated Allele Detail

Summary

• Symbol: Piwil4^em1Doca
• Name: piwi-like RNA-mediated gene silencing 4; endonuclease-mediated mutation 1, Donal O'Carroll
• MGI ID: MGI:6468283
• Synonyms: Miwi2^HA
• Gene: Piwil4 Location: Chr9:14613072-14651968 bp, - strand Genetic Position: Chr9, 4.25 cM
• Alliance: Piwil4^em1Doca page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Epitope tag)
• Mutation: Insertion
• Mutation details: Using an sgRNA targeting the start of the coding region and a DNA oligonucleotide template with CRISPR/Cas9 technology, HA epitope tag sequence was inserted immediately after the ATG start codon. This creates an allele that expresses a fully functional endogenous peptide with an N-terminal HA tag. (J:262370)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Piwil4 Mutation: 49 strains or lines available

References

• Original: J:262370 Vasiliauskaite L, et al., A MILI-independent piRNA biogenesis pathway empowers partial germline reprogramming. Nat Struct Mol Biol. 2017 Jul;24(7):604-606
• All: 5 reference(s)