Tet1^tm1.1Koh
Targeted Allele Detail

Summary

• Symbol: Tet1^tm1.1Koh
• Name: tet methylcytosine dioxygenase 1; targeted mutation 1.1, Kian Peng Koh
• MGI ID: MGI:6467253
• Gene: Tet1 Location: Chr10:62640349-62723242 bp, - strand Genetic Position: Chr10, 32.48 cM
• Alliance: Tet1^tm1.1Koh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:288065
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: A target vector was designed to insert an loxP-NLS-lacZ-loxP-mCherry-FRT-neo-FRT cassette (containing ATG start codon and nuclear localization signal sequence 5 of lacZ reporter gene with 3 BGH polyA, flanked by 2 LoxP sites to allow reporter exchange to a downstream ATG-mCherry-pA cassette by Cre recombination, followed by a FRT-flanked neomycin selection cassette) in-frame at the ATG start codon in exon 2. Flp-mediated recombination removed the selection cassette. (J:288065)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tet1 Mutation: 147 strains or lines available

References

• Original: J:288065 Bartoccetti M, et al., Regulatory Dynamics of Tet1 and Oct4 Resolve Stages of Global DNA Demethylation and Transcriptomic Changes in Reprogramming. Cell Rep. 2020 Feb 18;30(7):2150-2169.e9
• All: 1 reference(s)