Afdn^tm1.2Lfr
Targeted Allele Detail

Summary

• Symbol: Afdn^tm1.2Lfr
• Name: afadin, adherens junction formation factor; targeted mutation 1.2, Louis F Reichardt
• MGI ID: MGI:6466561
• Synonyms: Afadin^-
• Gene: Afdn Location: Chr17:13980735-14126059 bp, + strand Genetic Position: Chr17, 8.95 cM, cytoband A2
• Alliance: Afdn^tm1.2Lfr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:271772
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 2 and an FRT flanked neomycin selection cassette and second loxP site were inserted downstream of exon 2 via homologous recombination. Flp and Cre-mediated recombination resulted in the removal of the selection cassette and exon 2. (J:179366)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Afdn Mutation: 82 strains or lines available

References

• Original: J:271772 Dewitz C, et al., Nuclear Organization in the Spinal Cord Depends on Motor Neuron Lamination Orchestrated by Catenin and Afadin Function. Cell Rep. 2018 Feb 13;22(7):1681-1694
• All: 2 reference(s)