Ncr1^{em1(Klra1)Wyok}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ncr1^{em1(Klra1)Wyok}
• Name: natural cytotoxicity triggering receptor 1; endonuclease-mediated mutation 1, Wayne Yokoyama
• MGI ID: MGI:6460365
• Synonyms: Ly49A-KI
• Gene: Ncr1 Location: Chr7:4340723-4348163 bp, + strand Genetic Position: Chr7, 2.5 cM
• Alliance: Ncr1^{em1(Klra1)Wyok} page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Inserted expressed sequence)
• Mutation: Insertion
• Ncr1^{em1(Klra1)Wyok} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Ncr1 (MGI:1336212)	mouse cDNA inserted in-frame via P2A self-cleaving peptide linker, replacing stop codon and first 32 bp of 3' UTR

• Mutation details: Using CRISPR/Cas9 technology with two sgRNAs targeting the stop codon, a mouse Klra1 cDNA, preceded by sequence for the self-cleaving P2A peptide, was inserted. The insertion replaced the stop codon and the first 32 bp of the 3' UTR. (J:295190)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ncr1 Mutation: 36 strains or lines available

References

• Original: J:295190 Parikh BA, et al., Control of Viral Infection by Natural Killer Cell Inhibitory Receptors. Cell Rep. 2020 Jul 28;32(4):107969
• All: 1 reference(s)