Klra^em1Wyok
Endonuclease-mediated Allele Detail

Summary

• Symbol: Klra^em1Wyok
• Name: killer cell lectin-like receptor, subfamily A; endonuclease-mediated mutation 1, Wayne Yokoyama
• MGI ID: MGI:6460343
• Synonyms: deltaLy49-1
• Gene: Klra Location: unknown Genetic Position: Chr6, Syntenic

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutations: Intergenic deletion, Intragenic deletion
• Mutation details: Using CRISPR/Cas9 technology with a promiscuous sgRNA that binds to multiple cluster members, the following cluster members were wholly or partially deleted: Klra11-ps (5' end), Klra8 and Klra14-ps (3' end) (~66 kb deletion); Klra7 (5' end), Klra10, Klra13-ps, Klra3 and Klra1 (3' end) (~149 kb deletion). Flow cytometry confirmed the loss of expression from the protein coding genes. Expression of Klra4, which is not deleted, was significantly decreased, suggesting the deletions encompass locus control region sequences. (J:295190)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Klra Mutation: 0 strains or lines available

References

• Original: J:295190 Parikh BA, et al., Control of Viral Infection by Natural Killer Cell Inhibitory Receptors. Cell Rep. 2020 Jul 28;32(4):107969
• All: 1 reference(s)