Mir210^tm1.2Mtm
Targeted Allele Detail

Summary

• Symbol: Mir210^tm1.2Mtm
• Name: microRNA 210; targeted mutation 1.2, Michael McManus
• MGI ID: MGI:6459297
• Gene: Mir210 Location: Chr7:140801297-140801406 bp, - strand Genetic Position: Chr7, 86.52 cM
• Alliance: Mir210^tm1.2Mtm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:294951
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was designed to insert an FRT site followed by a lacZ gene, a loxP site, a neomycin cassette, a second FRT site and a second loxP site (FRT-lacZ-loxP-Neo-FRT-loxP) upstream of the microRNA stem loop and a third loxP site immediately downstream of the microRNA stem loop.The LacZ-neo cassette was removed through flp-mediated recombination and the stem loop was removed through subsequent cre-mediated recombination. (J:294951)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mir210 Mutation: 8 strains or lines available

References

• Original: J:294951 Krawczynski K, et al., Intact feto-placental growth in microRNA-210 deficient mice. Placenta. 2016 Nov;47:113-115
• All: 3 reference(s)