Hprt1^{tm2(ACTB-Bcl2)Sakb}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm2(ACTB-Bcl2)Sakb}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 2, Sarah K Bronson
• MGI ID: MGI:6459294
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm2(ACTB-Bcl2)Sakb} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:34887
• Parent Cell Line: BK4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Hprt1^{tm2(ACTB-Bcl2)Sakb} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Bcl2 (MGI:88138)	chicken promoter and mouse cDNA

• Mutation details: The following sequences were inserted in place of the endogenous promoter, exons 1-2 and 5' end of intron 2, which are lacking in the ES cells used: the chicken beta actin promoter, a mouse Bcl2 cDNA, PGK poly(A) signal sequence, human HPRT promoter and exon1, and mouse Hprt intron 1, exon 2 and part of intron 2. In this allele the chicken ACTB promoter drives the expression of murine Bcl2 and the human HPRT promoter drives the expression of the restored (but now human-mouse hybrid) Hprt gene. (J:34887)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1280 strains or lines available

References

• Original: J:34887 Bronson SK, et al., Single-copy transgenic mice with chosen-site integration [see comments]. Proc Natl Acad Sci U S A. 1996 Aug 20;93(17):9067-72
• All: 1 reference(s)