Tlr9^tm2.1Gbrt
Targeted Allele Detail

Summary

• Symbol: Tlr9^tm2.1Gbrt
• Name: toll-like receptor 9; targeted mutation 2.1, Gregory M Barton
• MGI ID: MGI:6459287
• Synonyms: TLR9^fsTM
• Gene: Tlr9 Location: Chr9:106099797-106104075 bp, + strand Genetic Position: Chr9, 57.46 cM
• Alliance: Tlr9^tm2.1Gbrt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:287989
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The targeting vector contains a loxP-flanked STOP cassette upstream of exon 2, an internal ribosome entry site (IRES) fused to a green fluorescent protein (GFP) sequence inserted downstream of exon 2, and a mutation was introduced in exon 2, in the transmembrane domain. The construct also contains an FRT-flanked neomycin resistance (neo) cassette. Flp-mediated recombination removed the FRT-flanked neo cassette. (J:287989)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tlr9 Mutation: 65 strains or lines available

References

• Original: J:287989 Stanbery AG, et al., Dysregulation of TLR9 in neonates leads to fatal inflammatory disease driven by IFN-gamma. Proc Natl Acad Sci U S A. 2020 Feb 11;117(6):3074-3082
• All: 1 reference(s)