Tg(CAG-EGFP,-mCherry,-Dnmt3a,-Dnmt3l)qYaob
Transgene Detail

Summary

• Symbol: Tg(CAG-EGFP,-mCherry,-Dnmt3a,-Dnmt3l)qYaob
• Name: transgene insertion q, Yayoi Obata
• MGI ID: MGI:6458805
• Synonyms: 2lox
• Transgene: Tg(CAG-EGFP,-mCherry,-Dnmt3a,-Dnmt3l)qYaob Location: unknown
• Alliance: Tg(CAG-EGFP,-mCherry,-Dnmt3a,-Dnmt3l)qYaob page

Transgene origin

• Strain of Origin: C57BL/6N

Transgene description

• Transgene Type: Transgenic (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Tg(CAG-EGFP,-mCherry,-Dnmt3a,-Dnmt3l)qYaob expresses 2 genes
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Dnmt3a (MGI:1261827)	mCherry, Dnmt3a and Dnmt3l are only expressed after cre-mediated recombination removes the EGFP gene
  mouse	Dnmt3l (MGI:1859287)	mCherry, Dnmt3a and Dnmt3l are only expressed after cre-mediated recombination removes the EGFP gene

• Mutation details: The transgene insert comprises the following elements: the CAG promoter, a loxP site, the EGFP fluorescent reporter gene, poly(A) signal sequence, a loxP site, the mCherry fluorescent reporter gene, sequence coding for T2A self-cleaving peptide, the cDNA sequence for the mouse Dnmt3a2 short splice variant, sequence coding for T2A self-cleaving peptide, mouse Dnmt3l cDNA sequence, and poly(A) signal sequence. This allele expresses the EGFP gene; mCherry, Dnmt3a and Dnmt3l are only expressed after cre-mediated recombination removes the EGFP gene. (J:210999)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:210999 Hara S, et al., Forced expression of DNA methyltransferases during oocyte growth accelerates the establishment of methylation imprints but not functional genomic imprinting. Hum Mol Genet. 2014 Jul 15;23(14):3853-64
• All: 1 reference(s)