Chmp4c^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Chmp4c^{tm1c(EUCOMM)Wtsi}
• Name: charged multivesicular body protein 4C; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6458055
• Gene: Chmp4c Location: Chr3:10432033-10456065 bp, + strand Genetic Position: Chr3, 2.63 cM, cytoband A2
• Alliance: Chmp4c^{tm1c(EUCOMM)Wtsi} page
• IMPC: Chmp4c gene page

Mutation origin

• Mutant Cell Line: EPD0113_1_B10
• Germline Transmission: Earliest citation of germline transmission: J:287102
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_gt1
• Mutation details: The L1L2_gt1 cassette was inserted at position 10450152 of Chromosome 3 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon 2 at position 10451009. The critical exon 2 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:287102)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Chmp4c Mutation: 21 strains or lines available

References

• Original: J:287102 Tedeschi A, et al., Cep55 promotes cytokinesis of neural progenitors but is dispensable for most mammalian cell divisions. Nat Commun. 2020 Apr 8;11(1):1746
• All: 2 reference(s)