Gli3^tm1.1Amc
Targeted Allele Detail

Summary

• Symbol: Gli3^tm1.1Amc
• Name: GLI-Kruppel family member GLI3; targeted mutation 1.1, Andrew P McMahon
• MGI ID: MGI:6457549
• Synonyms: Gli3^3xFLAG
• Gene: Gli3 Location: Chr13:15638308-15904611 bp, + strand Genetic Position: Chr13, 5.43 cM, cytoband A2
• Alliance: Gli3^tm1.1Amc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:213641
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Epitope tag)
• Mutation: Insertion
• Mutation details : Sequence for triple FLAG tags was inserted immediately downstream of the ATG translation start site, followed by sequence for an AviTag (GLNDIFEAQKIEWHE) and GSG linker sequence. The peptide encoded by this allele is thus tagged with N-terminal FLAG tags and contains an N-terminal biotinylation site that allows for streptavidin capture. The loxP site flanked neomycin resistance gene cassette that was also inserted was removed through subsequent cre-mediated recombination using mice carrying the Find Mice (IMSR):
  • Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
  • Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
  • Carrying any Gli3 Mutation: 84 strains or lines available
• References:
  • Original: J:213641 Lopez-Rios J, et al., Attenuated sensing of SHH by Ptch1 underlies evolution of bovine limbs. Nature. 2014 Jul 3;511(7507):46-51
  • All: 4 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 01/13/2026 MGI 6.24