Gt(ROSA)26Sor^{tm2(Tmem100)Spo}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm2(Tmem100)Spo}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 3, S Paul Oh
• MGI ID: MGI:6456071
• Synonyms: ROSA26^Tmem100
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm2(Tmem100)Spo} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:294364
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: The adenovirus splice acceptor sequence, the puromycin resistance gene (Puro), a CAGGS promoter, a loxP-flanked transcription stop cassette (tpA), the Tmem100 gene with the N-terminal HA-tag, an internal ribosome entry site, the eGFP gene, a polyadenylation sequence was inserted into the endogenous Gt(ROSA)26Sor locus. The Tmem100 expressed is induced by cre activity at high levels in most tissues of the body. (J:294364)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1083 strains or lines available

References

• Original: J:294364 Moon EH, et al., TMEM100 is a key factor for specification of lymphatic endothelial progenitors. Angiogenesis. 2020 Aug;23(3):339-355
• All: 1 reference(s)