Rock2^tm1.1Leiw
Targeted Allele Detail

Summary

• Symbol: Rock2^tm1.1Leiw
• Name: Rho-associated coiled-coil containing protein kinase 2; targeted mutation 1.1, Lei Wei
• MGI ID: MGI:6455469
• Synonyms: ROCK2^-
• Gene: Rock2 Location: Chr12:16944896-17037824 bp, + strand Genetic Position: Chr12, 8.04 cM, cytoband A3
• Alliance: Rock2^tm1.1Leiw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:294294
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A LoxP site was inserted into intron 1 and a second loxP site and an FRT site flanked neomycin resistance gene cassette into intron 2. Exon 2 was deleted through subsequent cre-mediated deletion (Tek-cre transgene). Southern blotting, PCR, and western blotting experiments confirmed deletion of the exon and lack of expression from the allele. (J:294294)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rock2 Mutation: 58 strains or lines available

References

• Original: J:294294 Shi J, et al., Distinct roles for ROCK1 and ROCK2 in the regulation of cell detachment. Cell Death Dis. 2013 Feb 7;4:e483
• All: 2 reference(s)