Lipa^{tm1d(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Lipa^{tm1d(EUCOMM)Hmgu}
• Name: lysosomal acid lipase A; targeted mutation 1d, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6454702
• Gene: Lipa Location: Chr19:34469718-34504874 bp, - strand Genetic Position: Chr19, 29.7 cM
• Alliance: Lipa^{tm1d(EUCOMM)Hmgu} page
• IMPC: Lipa gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:294097
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 34488106 of Chromosome 19 upstream of exon 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 4 at position 34489566. Exon 4 is thus flanked by loxP sites. The lacZ and neo cassettes were removed through subsequent flp-mediated recombination and exon 4 was deleted through cre-mediated recombination after that, creating a null allele. (J:294097)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lipa Mutation: 24 strains or lines available

References

• Original: J:294097 Wagner C, et al., Lysosomal acid lipase is the major acid retinyl ester hydrolase in cultured human hepatic stellate cells but not essential for retinyl ester degradation. Biochim Biophys Acta Mol Cell Biol Lipids. 2020 Aug;1865(8):158730
• All: 1 reference(s)