Tmc2^em1Mll
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tmc2^em1Mll
• Name: transmembrane channel-like gene family 2; endonuclease-mediated mutation 1, Ulrich Muller
• MGI ID: MGI:6452848
• Synonyms: Tmc2-MYC
• Gene: Tmc2 Location: Chr2:130037114-130106365 bp, + strand Genetic Position: Chr2, 63.19 cM
• Alliance: Tmc2^em1Mll page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Epitope tag)
• Mutation: Insertion
• Mutation details: Using CRISPR/Cas9 technology, sequence 5'-GAACAAAAACTCATCTCAGAAGAGGATCTG-3', encoding EQKLISEEDL, was inserted immediately before the endogenous stop codon, providing the encoded peptide with a C-terminal MYC tag. (J:293413)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tmc2 Mutation: 43 strains or lines available

References

• Original: J:293413 Cunningham CL, et al., TMIE Defines Pore and Gating Properties of the Mechanotransduction Channel of Mammalian Cochlear Hair Cells. Neuron. 2020 Jul 8;107(1):126-143.e8
• All: 2 reference(s)