Tg(H2-K^b-Tcrd57.9,Tcrg57.9)#Gks
Transgene Detail

Summary

• Symbol: Tg(H2-K^b-Tcrd57.9,Tcrg57.9)#Gks
• Name: transgene insertion, Gek-Kee Sim
• MGI ID: MGI:6451151
• Transgene: Tg(H2-K^b-Tcrd57.9,Tcrg57.9)#Gks Location: unknown
• Alliance: Tg(H2-K^b-Tcrd57.9,Tcrg57.9)#Gks page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: The rearranged Vgamma6-Jgammal-Cgammal Tcrg gene from mycrobacterial antigen-specific T cell hybridoma 57.9 was cut to remove the silencer element 3' of the Cgamma1 segment. The rearranged Vdelta1-Jdelta2-Cdelta Tcrd gene from the same hybridoma was cloned downstream of the H2-K^b promoter, followed by beta globin exon 2, intron 2, exon 3 and poly(A) signal and the Igh enhancer. The gamma and delta constructs were co-injected into fertilized eggs. Nine different transgenic mouse lines (52, 56, 74, 77, 78, 114, 115, 131, 134) were established. (J:166278)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:166278 Sim GK, et al., Commitment and maintenance of the alpha beta and gamma delta T cell lineages. J Immunol. 1995 Jun 1;154(11):5821-31
• All: 2 reference(s)