Gt(ROSA)26Sor^{tm4(CAG-mNeonGreen,-sfGFP)Xwy}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm4(CAG-mNeonGreen,-sfGFP)Xwy}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 4, X William Yang
• MGI ID: MGI:6451011
• Synonyms: MORF2
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm4(CAG-mNeonGreen,-sfGFP)Xwy} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:292704
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Epitope tag, Reporter)
• Mutation: Insertion
• Mutation details: The final vector, generated through site specific integrase-mediated transgenesis, contains from 5' to 3' attL,a CAG promoter, a loxP-flanked STOP cassette, a Kozak consensus start site (for strong initiation of translation), two Myc-tags, an optimized C22-repeat MORF switch, and a farnesylated mNeonGreen fluorescent reporter, a tdTomato linker domain sequence, a superfolder GFP fluorescent reporter with several V5 epitopes (smFP-V5), polyA, and attR-attP3-FRT-R1/2 inserted in to the Gt(ROSA)26Sor locus. mNeonGreen is one of the brightest green/yellow fluorescent proteins and is derived from Branchiostoma lanceolatum. The farnesylation sequence (CAAX domain from Ras) targets the reporter to membranous structures including neuronal dendrites and axons. sfGFP was altered to embed several V5 epitopes (V5 is a 14 amino acid simian virus 5 epitope) in the GFP scaffold - 3 epitopes at the N terminus, 4 epitopes into the internal 172-173 loop and 3 epitopes at the C terminus. The inclusion of the V5 epitopes creates a highly antigenic "spaghetti monster" fluorescent protein (smGFP) that contains numerous copies of peptide epitopes and simultaneously binds IgG antibodies at each location. (J:292704)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 944 strains or lines available

References

• Original: J:292704 Veldman MB, et al., Brainwide Genetic Sparse Cell Labeling to Illuminate the Morphology of Neurons and Glia with Cre-Dependent MORF Mice. Neuron. 2020 Aug 5
• All: 1 reference(s)