Ryr1^tm2.1Mfgo
Targeted Allele Detail

Summary

• Symbol: Ryr1^tm2.1Mfgo
• Name: ryanodine receptor 1, skeletal muscle; targeted mutation 2.1, Michael Goldberg
• MGI ID: MGI:6450020
• Synonyms: RC-deltaL
• Gene: Ryr1 Location: Chr7:28702765-28824599 bp, - strand Genetic Position: Chr7, 16.94 cM, cytoband A2-B3
• Alliance: Ryr1^tm2.1Mfgo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: iTL BF1 (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Humanized sequence)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: The targeting vector is designed to insert an FRT-flanked neomycin resistance gene upstream of exon 34, an amino acid substitution at position 1669 altering serine to cysteine (S1669C; TCG to TGC) and a Leu1716 deletion (CTC) into exon 34. A silent mutation Leu1715 (CTC to CTG) was introduced to create a Bcl1 enzyme site. The neo cassette was deleted by transient transfection of an expression vector of flp recombinase in the ES cells. (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ryr1 Mutation: 214 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 1 reference(s)