Marf1^em1Yqs
Endonuclease-mediated Allele Detail

Summary

• Symbol: Marf1^em1Yqs
• Name: meiosis regulator and mRNA stability 1; endonuclease-mediated mutation 1, You-Qiang Su
• MGI ID: MGI:6450000
• Synonyms: MARF1-eGFP
• Gene: Marf1 Location: Chr16:13927030-13977157 bp, - strand Genetic Position: Chr16, 9.69 cM
• Alliance: Marf1^em1Yqs page

Mutation origin

• Strain of Origin: (C57BL/6 x DBA/2)F1

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: CRIPSR/Cas9 genome editing technology was used to insert an eGFP cassette into the 3-end of the last exon of the gene, just before the stop codon. Western blot analysis showed that oocytes of the mice carrying this allele expressed a MARF1-eGFP fusion protein. (J:283570)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Marf1 Mutation: 79 strains or lines available

References

• Original: J:283570 Cao GY, et al., Interference with the C-terminal structure of MARF1 causes defective oocyte meiotic division and female infertility in mice. J Biomed Res. 2018 Jan 18;32(1):58-67
• All: 1 reference(s)