Dlg2^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Dlg2^{tm1d(EUCOMM)Wtsi}
• Name: discs large MAGUK scaffold protein 2; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:6449233
• Synonyms: Dlg2^delta14
• Gene: Dlg2 Location: Chr7:90125880-92098455 bp, + strand Genetic Position: Chr7, 51.07 cM
• Alliance: Dlg2^{tm1d(EUCOMM)Wtsi} page
• IMPC: Dlg2 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:287325
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 91934761 of Chromosome 7 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 91936081. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:287325)

Expression

In Mice Carrying this Mutation:

4 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dlg2 Mutation: 59 strains or lines available

References

• Original: J:287325 Yoo T, et al., A DLG2 deficiency in mice leads to reduced sociability and increased repetitive behavior accompanied by aberrant synaptic transmission in the dorsal striatum. Mol Autism. 2020 Mar 12;11(1):19
• All: 1 reference(s)